Coding
LysA

Part:BBa_K284001:Design

Designed by: Tais Sineiro Herig, Raissa Estrela Curado and Gleidson Silva Teixeira   Group: iGEM09_UNICAMP-Brazil   (2009-09-26)

Lysozyme from Gallus gallus



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 434
    Illegal NgoMIV site found at 477
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was built to be used in a Gram-positive bacteria lysis device.

Source

Total mRNA isolated from chicken oviduct was used for amplification of this part.

References

Vocadlo DJ, Davies GJ, Laine R and Withers SG. 2001. Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate. Nature 412, 835-838.